| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium tepidum | GCTTCTGTTTGCCCTGTGG | ACTCCACCACCGGACATTTC | 59.34 | 59.96 | 212 | 173 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium tepidum | GCTTCTGTTTGCCCTGTGG | AACTCCACCACCGGACAT | 59.34 | 58.10 | 213 | 149 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium tepidum | AGCTGGTAAGGGGTGTGAGG | CCACCAGAAACAGCTACAGC | 61.20 | 58.84 | 177 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium tepidum | GCTTCTGTTTGCCCTGTGG | TGTAACTCCACCACCGGAC | 59.34 | 58.95 | 216 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium tepidum | TGCAGATGAAGTTGGCGATTC | TCTGCCATTGGACACCCAG | 59.26 | 59.62 | 168 | 140 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium tepidum | TCTGCCTAGTGTTGGTATGCA | GTTTGGGAGTGACATGCCC | 59.09 | 58.73 | 268 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium tepidum | ACCCCAGTATGAACCGCA | TGTTGCTCAGTTCCTTTTGCA | 58.19 | 58.90 | 200 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium tepidum | AACCCCAGTATGAACCGCA | TGTTGCTCAGTTCCTTTTGCA | 58.93 | 58.90 | 201 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium tepidum | AAACCCCAGTATGAACCGCA | TGTTGCTCAGTTCCTTTTGCA | 59.60 | 58.90 | 202 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium tepidum | AAAACCCCAGTATGAACCGCA | TGTTGCTCAGTTCCTTTTGCA | 60.20 | 58.90 | 203 | 138 |
100.00%
|
100.00%
|